期刊
MICROBIOLOGY-SGM
卷 167, 期 12, 页码 -出版社
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.001123
关键词
sponge; natural product discovery; hexactinellid; antimicrobial; culture
类别
资金
- Society for Applied Microbiology
- School of Biological and Marine Sciences, University of Plymouth
Through culture-based microbial analysis of deep-sea sponges, this study discovered novel antimicrobial candidates and demonstrated antimicrobial activity, showcasing the potential for screening novel antimicrobial compounds.
Access to deep-sea sponges brings with it the potential to discover novel antimicrobial candidates, as well as novel cold- and pressure-adapted bacteria with further potential clinical or industrial applications. In this study, we implemented a combination of different growth media, increased pressure and high-throughput techniques to optimize recovery of isolates from two deep-sea hexactinellid sponges, Pheronema carpenteri and Hertwigia sp.. in the first culture-based microbial analysis of these two sponges. Using 16S rRNA gene sequencing for isolate identification, we found a similar number of cultivable taxa from each sponge species, as well as improved recovery of morphotypes from P carpenteri at 22-25 degrees C compared to other temperatures, which allows a greater potential for screening for novel antimicrobial compounds. Bacteria recovered under conditions of increased pressure were from the phyla Proteobacteria, Actinobacteria and Firmicutes, except at 4%O-2/5 bar, when the phylum Firmicutes was not observed. Cultured isolates from both sponge species displayed antimicrobial activity against Micrococcus luteus, Staphylococcus aureus and Escherichia coli.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据