4.7 Article

Transfer of plasmids harbouring blaOXA-48-like carbapenemase genes in biofilm-growing Klebsiella pneumoniae: Effect of biocide exposure

期刊

MICROBIOLOGICAL RESEARCH
卷 254, 期 -, 页码 -

出版社

ELSEVIER GMBH
DOI: 10.1016/j.micres.2021.126894

关键词

Carbapenemase-producing; K; pneumoniae; Biofilm; Biocides; Plasmid conjugation

资金

  1. Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III [PI15-01172]
  2. Subdireccion General de Redes y Centros de Investigacion Cooperativa
  3. Ministerio de Economia y Competitividad
  4. Spanish Network for Research in Infectious Diseases - European Development Regional Fund 'A way to achieve Europe' ERDF [REIPI RD12/0015]

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The study found that biofilm formation can affect the spread of OXA-48-Kpn, especially in high-risk clones. The presence of biocides reduced the fold changes in CF-BF/CF-PK in HR isolates, while it had minimal effect on non-HR isolates.
The spread of OXA-48-encoding plasmids from Klebsiella pneumoniae (OXA-48-Kpn), especially successful highrisk (HR) clones, is a growing concern. Biofilm formation can contribute to the dissemination of OXA-48-Kpn. It is not known whether biocides can affect the transfer of OXA-48-Kpn in biofilm. The aim of this study was to evaluate the effect of biocides on the conjugation frequency (CF) of OXA-48-Kpn in both biofilm and planktonic cultures. For that, seven OXA-48-Kpn isolates (4 belonging to HR clones and 3 to non-HR clones) were selected as donors. Each isolate was mixed (1:1) with Escherichia coli J53 (recipient) and grown on polystyrene microplates without biocides (control) and with 0.25x MIC of triclosan (TRI), chlorhexidine digluconate (CHX), povidone-iodine (POV), sodium hypochlorite (SOD) or ethanol (ETH). The CF was calculated as the number of transconjugants/number of E. coli J53. The results showed that for isolates growing in the absence of biocide, the mean fold change in the CF in biofilm with respect to that determined in planktonic cells (CF-BF/CF-PK) was 0.2 in non-HR isolates and ranged from 2.0 to 14.7 in HR isolates. In HR isolates grown in the presence of biocide, especially CHX, TRI, and ETH, the fold changes in CF-BF/CF-PK decreased, whereas in non-HR isolates the fold changes were similar or increased slightly with CHX, ETH, SOD and POV. In conclusion, the fold changes in the CF-BF/CF-PK are higher in HR isolates comparing to non-HR isolates in abscence of biocides. The fold changes in CF-BF/CF-PK of the HR isolates in the presence of biocides varied with the type of biocides, whereas in non-HR isolates, biocides have no significant effect, or produce only a slight increase in the fold change of CF-BF/CF-PK.

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