Hierarchical graph representations in digital pathology

Cancer diagnosis, prognosis, and therapy response predictions from tissue specimens highly depend on the phenotype and topological distribution of constituting histological entities. Thus, adequate tissue representations for encoding histological entities is imperative for computer aided cancer patient care. To this end, several approaches have leveraged cell-graphs, capturing the cell-microenvironment, to depict the tissue. These allow for utilizing graph theory and machine learning to map the tissue representation to tissue functionality, and quantify their relationship. Though cellular information is crucial, it is incomplete alone to comprehensively characterize complex tissue structure. We herein treat the tissue as a hierarchical composition of multiple types of histological entities from fine to coarse level, capturing multivariate tissue information at multiple levels. We propose a novel multi-level hierarchical entity graph representation of tissue specimens to model the hierarchical compositions that encode histological entities as well as their intra-and inter-entity level interactions. Subsequently, a hierarchical graph neural network is proposed to operate on the hierarchical entity-graph and map the tissue structure to tissue functionality. Specifically, for input histology images, we utilize well-defined cells and tissue regions to build HierArchical Cell-to-Tissue ( HACT ) graph representations, and devise HACT-Net, a message passing graph neural network, to classify the HACT representations. As part of this work, we introduce the BReAst Carcinoma Subtyping (BRACS) dataset, a large cohort of Haematoxylin & Eosin stained breast tumor regions-of-interest, to evaluate and benchmark our proposed methodology against pathologists and state-of-the-art computer-aided diagnostic approaches. Through comparative assessment and ablation studies, our proposed method is demonstrated to yield superior classification results compared to alternative methods as well as individual pathologists. The code, data, and models can be accessed at https://github.com/histocartography/hact-net . (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

The accurate diagnosis, prognosis, and therapy response predictions for cancer patients rely heavily on the phenotype and distribution of histological entities in tissue specimens. Various methods have been developed to represent tissue structures using cell-graphs, leveraging graph theory and machine learning. This study proposes a novel hierarchical entity graph representation for tissue specimens and introduces a hierarchical graph neural network to map tissue structure to functionality. Through evaluation with the BRACS dataset, the proposed method demonstrates superior classification results compared to alternative methods and individual pathologists.