期刊
MEDIATORS OF INFLAMMATION
卷 2022, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2022/8457010
关键词
-
资金
- Medical and Health Research Program of Zhejiang Province (Youth talent project) [2019RC038]
- National Natural Science Foundation of China [81901941, 81971871]
Glibenclamide alleviates the development of LPS-induced acute lung injury in a mouse model by inhibiting the NLRP3/Caspase-1/IL-1β signaling pathway.
Glibenclamide displays an anti-inflammatory response in various pulmonary diseases, but its exact role in lipopolysaccharide- (LPS-) induced acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) remains unknown. Herein, we aimed to explore the effect of glibenclamide in vivo and in vitro on the development of LPS-induced ALI in a mouse model. LPS stimulation resulted in increases in lung injury score, wet/dry ratio, and capillary permeability in lungs, as well as in total protein concentration, inflammatory cells, and inflammatory cytokines including IL-1 beta, IL-18 in bronchoalveolar lavage fluid (BALF), and lung tissues, whereas glibenclamide treatment reduced these changes. Meanwhile, the increased proteins of NLRP3 and Caspase-1/p20 after LPS instillation in lungs were downregulated by glibenclamide. Similarly, in vitro experiments also found that glibenclamide administration inhibited the LPS-induced upregulations in cytokine secretions of IL-1 beta and IL-18, as well as in the expression of components in NLRP3 inflammasome in mouse peritoneal macrophages. Of note, glibenclamide had no effect on the secretion of TNF-alpha in vivo nor in vitro, implicating that its anti-inflammatory effect is relatively specific to NLRP3 inflammasome. In conclusion, glibenclamide alleviates the development of LPS-induced ALI in a mouse model via inhibiting the NLRP3/Caspase-1/IL-1 beta signaling pathway, which might provide a new strategy for the treatment of LPS-induced ALI.
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