期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2021.112442
关键词
MgO nano-particles; Copper; Osteoblasts; Osteoclasts; bacteria
资金
- Na-tional Natural Science Foundation of China [51771233, 52071346, 51604104]
- China Postdoctoral Science Foundation [2018M633164]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515110736]
- Innovation-oriented Advanced Technology and Industrial Technology Program Project of Hunan Province [2020SK2017]
This study demonstrated the precise loading of Cu2+ onto MgO nanoparticles to create nano-composites with multi-functional properties for enhancing osteogenesis, inhibiting osteoclastogenesis, and effectively removing bacteria. The amount of Cu2+ loaded onto the MgO nanoparticles determined the biological responses of osteoblasts, osteoclasts, and bacteria, making MgO-xCu (x = 0.1, 1, 10) nano-composites promising for bone tissue engineering applications.
Biomaterials with multi-functions including enhancing osteogenesis, inhibiting osteoclastogenesis and effectively removing bacteria are urgently needed in the treatment of osteoporotic bone defects. In this study, MgO nano-particles were employed as a platform for precise Cu2+ loading. By immersing MgO into CuSO4 solution with a pre-defined concentration (0.1, 1 or 10 mM), 1 mg MgO adsorbed 3.25, 32.5 or 325 mu g Cu2+ from the solution. As-synthesized nano-composites were referred as MgO-0.1Cu, MgO-1Cu or MgO-10Cu depending on the concentration of employed CuSO4 solution. The results revealed that MgO-xCu (x = 0.1, 1 and 10) nano-composites were lamella-shaped and composed of amorphous Cu(OH)(2), crystalline Mg(OH)(2) and minor MgO. The extra-cellular release of Cu2+ was rather limited due the capture of Cu2+ by Mg(OH)(2). In vitro results revealed that MgO-xCu (x = 0.1, 1 and 10) nano-composites modulated osteoblast, osteoclast and bacterium response in a Cu2+ loading amount-dependent manner. MgO-0.1Cu nano-composite exhibited stimulatory function on osteo-blast proliferation without influencing osteoblast maturation, osteoclast formation and bacterial survival. MgO-1Cu nano-composite enhanced osteoblast proliferation and differentiation, inhibited osteoclast formation and effectively killed bacteria. When larger amount of Cu2+ was loaded, MgO-10Cu nano-composite exhibited stronger stimulatory effect on osteoblast maturation, enhanced inhibitory function on osteoclast formation and promoted bactericidal performance, although it showed a certain degree of initial cyto-toxicity. Together, the results suggest that MgO nano-particles could be employed as potential platform for precise Cu2+ loading and intracellular Cu2+ delivery. MgO-xCu (x = 1 and 10) nano-composites are promising to be employed as multi-functional fillers in bone tissue engineering scaffolds for osteoporotic bone regeneration.
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