期刊
INDOOR AIR
卷 27, 期 2, 页码 345-353出版社
WILEY
DOI: 10.1111/ina.12306
关键词
16S; Airborne; Bacteria; Cooling coil-associated microbial communities; Fungi; HVAC systems
类别
资金
- Singapore Ministry of Education Academic Research Fund Tier 3 [MOE2013-T3-1-013]
- Singapore Centre on Environmental Life Sciences Engineering (SCELSE) - National Research Foundation Singapore
- Ministry of Education
- Nanyang Technological University
- National University of Singapore, under its Research Centre of Excellence Program
Air-conditioning systems harbor microorganisms, potentially spreading them to indoor environments. While air and surfaces in airconditioning systems are periodically sampled as potential sources of indoor microbes, little is known about the dynamics of cooling coil-associated communities and their effect on the downstream airflow. Here, we conducted a 4-week time series sampling to characterize the succession of an air-conditioning duct and cooling coil after cleaning. Using an universal primer pair targeting hypervariable regions of the 16S/18S ribosomal RNA, we observed a community succession for the condensed water, with the most abundant airborne taxon Agaricomycetes fungi dominating the initial phase and Sphingomonas bacteria becoming the most prevalent taxa toward the end of the experiment. Duplicate air samples collected upstream and downstream of the coil suggest that the system does not act as ecological filter or source/sink for specific microbial taxa during the duration of the experiment.
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