4.7 Article

Effect of Different Initial Fermentation pH on Exopolysaccharides Produced by Pseudoalteromonas agarivorans Hao 2018 and Identification of Key Genes Involved in Exopolysaccharide Synthesis via Transcriptome Analysis

期刊

MARINE DRUGS
卷 20, 期 2, 页码 -

出版社

MDPI
DOI: 10.3390/md20020089

关键词

marine bacteria; structural analysis; transcriptome sequence; KEGG analysis; GO analysis

资金

  1. Foundation of State Key Laboratory of Biobased Material [ZZ20190302]
  2. Foundation of Shandong Provincial Key Laboratory of Biosensors [SWCG 2018-01]
  3. Natural Science Foundation of Shandong Province [ZR2012CM019]
  4. Foundation [202002]
  5. Qilu University of Technology of Cultivating Subject for Biology and Biochemistry, Science, Education and Industry Integration Innovation Pilot Project of Qilu University of Technology (Shandong Academy of Sciences) [2020KJC-ZD08]

向作者/读者索取更多资源

This study investigates the effects of different initial fermentation pH levels on the production and composition of exopolysaccharides (EPSs) in Pseudoalteromonas agarivorans Hao 2018. The transcriptome analysis reveals that genes involved in EPS synthesis are regulated by changes in environmental pH. These findings contribute to understanding the environmental adaptation mechanisms of P. agarivorans and provide guidance for the development of new products using biologically active polysaccharides.
Exopolysaccharides (EPSs) are carbohydrate polymers produced and secreted by microorganisms. In a changing marine environment, EPS secretion can reduce damage from external environmental disturbances to microorganisms. Meanwhile, EPSs have promising application prospects in the fields of food, cosmetics, and pharmaceuticals. Changes in external environmental pH have been shown to affect the synthesis of EPSs in microorganisms. In this study, we analyzed the effects of different initial fermentation pHs on the production, monosaccharide composition, and antioxidant activity of the EPSs of Pseudoalteromonas agarivorans Hao 2018. In addition, the transcriptome sequence of P. agarivorans Hao 2018 under different initial fermentation pH levels was determined. GO and KEGG analyses showed that the differentially expressed genes were concentrated in the two-component regulatory system and bacterial chemotaxis pathways. We further identified the expression of key genes involved in EPS synthesis during pH changes. In particular, the expression of genes encoding the glucose/galactose MFS transporter, phosphomannomutase, and mannose-1-phosphate guanylyltransferase was upregulated when the environmental pH increased, thus promoting EPS synthesis. This study not only contributes to elucidating the environmental adaptation mechanisms of P. agarivorans, but also provides important theoretical guidance for the directed development of new products using biologically active polysaccharides.

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