期刊
MARINE DRUGS
卷 19, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/md19120653
关键词
Saccharomyces cerevisiae; Phaeodactylum tricornutum; Sparus aurata; beta-glucans; pulse feeding; immune tolerance
资金
- Bio Based Industries Joint Undertaking (BBI JU) under the European Union's Horizon 2020 research and innovation programme [745754]
- Norte Portugal Regional Operational Programme (NORTE 2020) under the PORTUGAL 2020 [NORTE-01-0145-FEDER-000040]
- European Regional Development Fund (ERDF)
- FCT-Foundation for Science and Technology [UIDB/04423/2020, UIDP/04423/2020, PD/BDE/129262/2017, IF/00197/2015]
- Fundação para a Ciência e a Tecnologia [PD/BDE/129262/2017] Funding Source: FCT
The study evaluated the effects of dietary supplementation with beta-glucans from yeast and microalga on gene expression, oxidative stress biomarkers, and plasma immune parameters in gilthead seabream juveniles. The results suggest that a P. tricornutum 37% enriched-beta-glucans extract may serve as a counter-measure in the context of gut inflammation, due to its immune-tolerant and anti-oxidative effects.
This work aimed to evaluate the effects of dietary supplementation with beta-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different beta-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived beta-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% beta-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 & PLUSMN; 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed beta-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-beta-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects.
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