4.6 Article

MiRNA-mRNA Integration Analysis Reveals the Regulatory Roles of MiRNAs in Shell Pigmentation of the Manila clam (Ruditapes philippinarum)

期刊

MARINE BIOTECHNOLOGY
卷 23, 期 6, 页码 976-993

出版社

SPRINGER
DOI: 10.1007/s10126-021-10080-1

关键词

Ruditapes philippinarum; Shell color; Pigmentation; miRNAGreek ano teleia mRNA

资金

  1. Chinese Ministry of Science and Technology through the National Key Research and Development Program of China [2018YFD0901400, 2019YFD0900704]
  2. China Agriculture Research System of MOF
  3. Liaoning BaiQianWan Talents Program
  4. Intercollegiate jointtraining program of colleges and universities in Liaoning Province
  5. Outstanding Chinese and Foreign Youth Exchange Program of China Association for Science and Technology (CAST)
  6. China Agriculture Research System of MARA

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The study revealed that shell color formation and regulation in the Manila clam is negatively regulated by multiple miRNAs, which may affect melanin formation and participate in the calcium signaling pathway. Verification of candidate miRNAs and target genes in different shell color groups suggested that miR-7, miR-71, and miR-133 may play important roles in shell color pigmentation.
The shell color of the Manila clam (Ruditapes philippinarum) is an economically important trait. We used high-throughput sequencing and transcriptome analysis to study the molecular mechanisms that underlie shell color formation and regulation in this species. We constructed small RNA libraries from mantle tissues from four shell color strains of Manila clam, subjected them to high-throughput sequencing. Notably, the results suggested that a number of pigment-associated genes including Mitf, HERC2, were negatively regulated by nvi-miR-2a, tgu-miR-133-3p, respectively. They might be involved in melanin formation via the activation of the melanogenesis pathway. And aae-miR-71-5p and dme-miR-7-5p linked to shell formation-related genes such as Calmodulin and IMSP3 were considered to participate in the calcium signaling pathway. We then used quantitative PCR to verify the candidate miRNAs and target genes in different shell color groups. Our results indicated that miR-7, miR-71, and miR-133 may regulate target mRNAs to participate in shell color pigmentation. These results provide the foundation to further characterize miRNA effects on the regulation of shell color and have significant implications for the breeding of new varieties of clams.

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