期刊
MACROMOLECULAR BIOSCIENCE
卷 22, 期 3, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.202100435
关键词
3D bioprinting; bioinks; cartilage; chondroitin sulfate; dermatan sulfate; tissue engineering
资金
- Basque Government [PRE_2020_2_0143]
- Basque Country Government [IT907-16]
- Ministerio de Economia, Industria y Competitividad (FEDER funds) [RTC-2016-5451-1]
- Fundacion Mutua Madrilena [FMM-AP171962019]
- Consejeria de Economia, Conocimiento, Empresas y Universidad de la Junta de Andalucia (ERDF funds) [B-CTS-230-UGR18, SOMM17-6109, P18-FR-2465]
- Instituto de Salud Carlos III, ERDF funds [DTS19/00145, DTS21/00098]
- Spanish Ministry of Science and Innovation (MICINN) [PID2020114086RB-100]
This study focuses on the development of nanocellulose-alginate based bioinks for 3D bioprinting for cartilage regeneration. The addition of chondroitin sulfate and dermatan sulfate improves the characteristics of the ink and promotes differentiation to cartilage.
Cartilage is a connective tissue which a limited capacity for healing and repairing. In this context, osteoarthritis (OA) disease may be developed with high prevalence in which the use of scaffolds may be a promising treatment. In addition, three-dimensional (3D) bioprinting has become an emerging additive manufacturing technology because of its rapid prototyping capacity and the possibility of creating complex structures. This study is focused on the development of nanocellulose-alginate (NC-Alg) based bioinks for 3D bioprinting for cartilage regeneration to which it is added chondroitin sulfate (CS) and dermatan sulfate (DS). First, rheological properties are evaluated. Then, sterilization effect, biocompatibility, and printability on developed NC-Alg-CS and NC-Alg-DS inks are evaluated. Subsequently, printed scaffolds are characterized. Finally, NC-Alg-CS and NC-Alg-DS inks are loaded with murine D1-MSCs-EPO and cell viability and functionality, as well as the chondrogenic differentiation ability are assessed. Results show that the addition of both CS and DS to the NC-Alg ink improves its characteristics in terms of rheology and cell viability and functionality. Moreover, differentiation to cartilage is promoted on NC-Alg-CS and NC-Alg-DS scaffolds. Therefore, the utilization of MSCs containing NC-Alg-CS and NC-Alg-DS scaffolds may become a feasible tissue engineering approach for cartilage regeneration.
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