Impact of Cell Loading of Recombinant Spider Silk Based Bioinks on Gelation and Printability

Printability of bioinks encompasses considerations concerning rheology and extrudability, characterization of filament formation, shape fidelity, cell viability, and post-printing cellular development. Recombinant spider silk based hydrogels might be a suitable material to be used in bioinks, that is, a formulation of cells and materials to be used for bioprinting. Here, the high shape fidelity of spider silk ink is shown by bioprinting the shape and size of a human aortic valve. Further the influence of the encapsulation of cells has been evaluated on spider silk hydrogel formation, hydrogel mechanics, and shape fidelity upon extrusion based bioprinting. It is shown that the presence of cells impacts the gelation of spider silk proteins differently, depending on the used silk variant. RGD-modified spider silk hydrogels are physically crosslinked by the cells, while there is no active interaction between cells and un-tagged spider silk proteins. Strikingly, even at cell densities up to ten million cells per milliliter, cell viability is high after extrusion-based printing, which is a significant prerequisite for future applications. Shape fidelity of the printed constructs is demonstrated using a filament collapse test in the absence and presence of human cells.

Automated summary

The printability of bioinks refers to considerations such as rheology, extrudability, filament formation, shape fidelity, cell viability, and cellular development post-printing. Recent studies indicate that recombinant spider silk hydrogels show high shape fidelity and may be suitable for bioink formulations, with the encapsulation of cells impacting gelation and mechanics differently depending on the silk variant used. RGD-modified spider silk hydrogels are physically crosslinked by cells, showing high cell viability after extrusion-based printing.