期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 153, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.112345
关键词
Bioactivities; Processing characteristics; Exopolysaccharides; Lactic acid bacteria; Plant-based protein gel
资金
- Natural Science Foundation of Heilongjiang Province [YQ2019C010]
The application of plant-based protein gels induced by lactic acid bacteria fermentation is closely related to the bioactivities and processing characteristics of exopolysaccharide (EPS). Among the EPS produced by different strains of Lactobacillus, CEPS showed the highest inhibition rates against various enzymes and possessed the highest water solubility index and water holding capacity. The EPS had high melting points, and their flow behavior and temperature-dependent behavior were well fitted with specific models, while CEPS had high negative charge intensity as supported by zeta potential, which could be used to evaluate bioactivities.
The application of plant-based protein gels induced by lactic acid bacteria (LAB) fermentation is closely related to bioactivities and processing characteristics of exopolysaccharide (EPS). EPS produced by L. acidophilus (AEPS), L. casei (CEPS), L. mesenteroides (MEPS), and L. lactis (LEPS) were studied. CEPS showed the highest inhibition rates against alpha-amylase (55.47 +/- 1.59%), alpha-glucosidase (60.47 +/- 0.89%), HT-29 (70.68 +/- 1.60%) and Caco-2 (60.80 +/- 1.58%), and CEPS possessed the highest water solubility index (20.78 +/- 1.92%) and water holding capacity (300.67 +/- 10.19%). Thermal analysis revealed that EPS had melting points from 237.04 to 241.72 degrees C. The flow behavior and temperature-dependent behavior were fitted well with the Power Law model and Arrhenius model, respectively (R-2 > 0.98). CEPS had more negative charge than other EPS, supported by zeta potential. Charge intensity was one of the mechanisms to regulate the EPS's bioactivities, and zeta potential could be used to evaluate its bioactivities.
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