期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 152, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.112313
关键词
Sufu; Flavor composition; Microbial diversity; Fermented food
资金
- National Key Research and Development Project [2018YFE0206300]
- Research Team Project of Natural Science Foundation of Heilongjiang Province [TD2020C003]
- Advantage and Characteristic of Discipline of Coarse Production and Processing in Heilongjiang Province [[2018]4]
- Heilongjiang Natural Science Foundation Project [LH2019C049, QC2017024]
- Innovative Talents Project of Heilongjiang Bayi Agricultural University [CXRC2017010]
This study used high-throughput sequencing and chemical analysis to reveal the microbial succession and metabolite changes during the fermentation stages of CS sufu production, showing significant differences in microbial communities and enriched flavor compounds at different stages. Sweet and umami amino acids predominantly contributed to the taste, while esters accounted for the highest proportion of flavor compounds.
CS sufu is a fermented food composed of mixed chickpeas and soybeans. To explore the effects of the microbial community on the physicochemical and flavor properties of this product, microbial succession and metabolite changes during the fermentation stage of CS sufu production were detected using high-throughput sequencing and HS-SPME-GC-MS. The results showed that bacterial diversity was higher than that of fungi in CS sufu, and core communities included Enterococcus, Enterobacter and Rhizopus. Moreover, enriched species in different fermentation stages were significantly different. Seventeen free amino acids (FAAs) were detected at the post fermentation stage. Sweet amino acids (TSAAs) and umami amino acids (TUAAs) mainly contributed to the taste of CS sufu. A total of 106 flavor compounds were identified at the different post-fermentation stages of CS sufu production, and esters, including ethyl caproate, ethyl octanoate, and isoamyl phenylacetate, accounted for the highest proportion. Furthermore, metabolic profile and microorganism analyses showed a significant correlation between the microbial profile and dominant flavor compounds. Lactococcus and Enterococcus were positively correlated with flavor compounds. This study provided information for the analysis of CS sufu at different fermentation periods in terms of microbial diversity and metabolites, and this information is important for understanding the properties of sufu made with mixed soybeans.
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