Understanding the Adhesion Mechanism of Hydroxyapatite-Binding Peptide

Understanding the interactions between the protein collagen and hydroxyapatite is of high importance for understanding biomineralization and bone formation. Here, we undertook a reductionist approach and studied the interactions between a short peptide and hydroxyapatite. The peptide was selected from a phagedisplay library for its high affinity to hydroxyapatite. To study its interactions with hydroxyapatite, we performed an alanine scan to determine the contribution of each residue. The interactions of the different peptide derivatives were studied using a quartz crystal microbalance with dissipation monitoring and with single-molecule force spectroscopy by atomic force microscopy. Our results suggest that the peptide binds via electrostatic interactions between cationic moieties of the peptide and the negatively charged groups on the crystal surface. Furthermore, our findings show that cationic residues have a crucial role in binding. Using molecular dynamics simulations, we show that the peptide structure is a contributing factor to the adhesion mechanism. These results suggest that even small conformational changes can have a significant effect on peptide adhesion. We suggest that a bent structure of the peptide allows it to strongly bind hydroxyapatite. The results presented in this study improve our understanding of peptide adhesion to hydroxyapatite. On top of physical interactions between the peptide and the surface, peptide structure contributes to adhesion. Unveiling these processes contributes to our understanding of more complex biological systems. Furthermore, it may help in the design of de novo peptides to be used as functional groups for modifying the surface of hydroxyapatite.

Automated summary

Understanding the interactions between collagen and hydroxyapatite is crucial for biomineralization and bone formation. In this study, the researchers investigated the interactions between a short peptide and hydroxyapatite. They found that the peptide binds to hydroxyapatite through electrostatic interactions and that cationic residues play a crucial role in the binding. Molecular dynamics simulations revealed that the peptide structure is also important for adhesion. These findings enhance our understanding of peptide adhesion to hydroxyapatite and have potential implications in designing functional groups for modifying hydroxyapatite surfaces.