期刊
KIDNEY INTERNATIONAL
卷 101, 期 5, 页码 1003-1016出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.kint.2021.12.027
关键词
antibody-mediated rejection; B cells; kidney transplantation; regulatory lymphocytes; T cells
资金
- National Institutes of Health [R21-AI116746, R01 AI130010, 5T32AI074490-12]
- Human Immunology Program at the STI
This study used high dimensional flow cytometry to analyze the status of regulatory T cells and transitional B cells in kidney transplant recipients. It found that in patients with antibody-mediated rejection (ABMR), both cell types were significantly reduced in numbers, which correlated with inflammatory antibody responses, microvascular inflammation, and kidney allograft loss.
Although considerable advances have been made in understanding the cellular effector mechanisms responsible for donor-specific antibody generation leading to antibody-mediated rejection (ABMR), the identification of cellular regulators of such immune responses is lacking. To clarify this, we used high dimensional flow cytometry to concomitantly profile and track the two major subsets of regulatory lymphocytes in blood: T regulatory (TREG) and transitional B cells in a cohort of 96 kidney transplant recipients. Additionally, we established co-culture assays to address their respective capacity to suppress antibody responses in vitro. TREG and transitional B cells were found to be potent suppressors of T follicular helper-mediated Bcell differentiation into plasmablast and antibody generation. TREG and transitional B cells were both durably expanded in patients who did not develop donor-specific antibody post-transplant. However, patients who manifested donor-specific antibody and progressed to ABMR displayed a marked and persistent numerical reduction in TREG and transitional B cells. Strikingly, specific cell clusters expressing the transcription factor Tbet were selectively depleted in both TREG and transitional B-cell compartments in patients with ABMR. Importantly, the coordinated loss of these T-bet(+)CXCR5(+)TREG and T-bet(+)CD21(-) transitional B-cell clusters was correlated with increased and inflammatory donor specific antibody responses, more extensive microvascular inflammation and a higher rate of kidney allograft loss. Thus, our study identified coordinated and persistent defects in regulatory T- and B-cell responses in patients undergoing ABMR, which may contribute to their loss of humoral immune regulation, and warrant timely therapeutic interventions to replenish and sustain TREG and transitional B cells in these patients.
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