Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

Automated summary

The COVID-19 pandemic has shown the importance of generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers have been optimized to reduce cross-well contamination and maintain sensitivity.