期刊
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
卷 102, 期 6, 页码 2598-2603出版社
WILEY
DOI: 10.1002/jsfa.11601
关键词
black soldier fly larva; compost; fungal community; ITS metabarcoding; yeasts
资金
- Ministry of Science and Higher Education of the Russian Federation [075-11-2019-070]
- Russian Science Foundation [19-74-10002]
- Russian Science Foundation [19-74-10002] Funding Source: Russian Science Foundation
Black soldier fly larvae (BSFL) show promise in producing cheap and high-quality dietary protein. This study investigated the fungal community of compost produced by BSFL larvae reared on a food waste substrate. The final mycobiome of the compost consisted of only two yeast species, with a total abundance of 1.2 x 10(7) CFU g(-1), after the incubation of larvae.
BACKGROUND Black soldier fly larvae (BSFL) offer very good prospects for the production of cheap and high-quality dietary protein. This insect is able to consume low-quality substrates, including food waste. The properties and safety of the compost obtained are largely determined by its microbiome. However, while the bacterial component of the BSFL-compost microbiome has been much studied, little is known about its fungal component. In plant-based rearing substrates both the biomass and the metabolic activity of fungi often exceed the biomass and the activity of prokaryotes. So, the purpose of this study was to investigate the fungal community of the compost produced by BSF larvae reared on a food waste substrate. RESULTS Community structure was determined by metabarcoding of the ITS (internal transcribed spacer) region. Species composition and abundance were determined using the plating technique and subsequent identification of the isolated pure cultures. It was found that the primary mycobiome of the food waste substrate that was used consisted of 19 families, represented mainly by phytopathogenic and endophytic genera. Larva incubation led to the complete elimination of all mycelial fungi from the resulting compost. The final mycobiome consisted of only two yeast species, Pichia kudriavzevii and Diutina rugosa, with a total abundance of 1.2 x 10(7) CFU g(-1). CONCLUSIONS The rearing of BSFL led to the complete elimination of mycelial fungi from its feed substrate. The final compost lacked harmful fungi, including molds. This information may be crucial for BSF compost utilization. The phenomenon was also an interesting aspect of zoomicrobial interactions in nature and agriculture. (c) 2021 Society of Chemical Industry.
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