期刊
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
卷 33, 期 2, 页码 279-289出版社
AMER SOC NEPHROLOGY
DOI: 10.1681/ASN.2021081150
关键词
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资金
- Ruth L. Kirschstein National Research Service Award (NIDDK) [F32DK130249, UC2DK126024]
- Washington University-Centene ARCH Personalized Medicine Initiative [P19-00559]
This study utilized a new spatial transcriptomic sequencing platform to analyze the topography of gene expression and revealed intercellular communication networks and regional gene expression patterns during kidney injury and repair. The results showed that injured proximal tubule cells continued to interact with macrophages and lymphocytes even 6 weeks after injury, potentially reflecting the transition from acute kidney injury to chronic kidney disease.
Background Single-cell sequencing technologies have advanced our understanding of kidney biology and disease, but the loss of spatial information in these datasets hinders our interpretation of intercellular communication networks and regional gene expression patterns. New spatial transcriptomic sequencing platforms make it possible to measure the topography of gene expression at genome depth. Methods We optimized and validated a female bilateral ischemia-reperfusion injury model. Using the 103 Genomics Visium Spatial Gene Expression solution, we generated spatial maps of gene expression across the injury and repair time course, and applied two open-source computational tools, Giotto and SPOTlight, to increase resolution and measure cell-cell interaction dynamics. Results An ischemia time of 34 minutes in a female murine model resulted in comparable injury to 22 minutes for males. We report a total of 16,856 unique genes mapped across our injury and repair time course. Giotto, a computational toolbox for spatial data analysis, enabled increased resolution mapping of genes and cell types. Using a seeded nonnegative matrix regression (SPOTlight) to deconvolute the dynamic landscape of cell-cell interactions, we found that injured proximal tubule cells were characterized by increasing macrophage and lymphocyte interactions even 6 weeks after injury, potentially reflecting the AKI to CKD transition. Conclusions In this transcriptomic atlas, we defined region-specific and injuryinduced loss of differentiation markers and their re-expression during repair, as well as region-specific injury and repair transcriptional responses. Lastly, we created an interactive data visualization application for the scientific community to explore these results (http://humphreyslab.com/SingleCell/).
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