期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 33, 期 1, 页码 11-16出版社
AMER CHEMICAL SOC
DOI: 10.1021/jasms.1c00335
关键词
MALDI; mass spectrometry imaging; matrix deposition; protein; brain
资金
- Shandong Provincial Key Research and Development Program (Major Scientific and Technological Innovation Project) [2019JZZY020903]
- National Natural Science Foundation of China [22074071]
- Research Grants Council of Hong Kong [4053386]
- Program for Taishan Scholars of Shandong Province [tsqn202103099, tsqn201812088]
A new integrated matrix deposition device with synchronous solvent nebulization was developed for MALDI-mass spectrometry imaging, improving efficiency and quality in imaging of protein and small metabolite distributions in mouse brain tissue sections. The device demonstrated enhanced sensitivity and image quality compared to conventional methods, showing its utility in MALDI-MSI applications.
The current matrix deposition methods in MALDI-mass spectrometry imaging (MALDI-MSI) face technical problems related to the inhomogeneous distribution of crystals and the low analyte extraction and cocrystallization efficiency. In this work, an integrated matrix sublimation device with synchronous solvent nebulization was developed for MALDI-MSI. Droplets of solvents were directly introduced into the chamber of the sublimator by using a miniaturized ultrasonic nebulizer unit. The synchronous and asynchronous working modes of solvent nebulization and matrix sublimation were systematically investigated. Imaging of both protein and small metabolite distributions in mouse brain tissue sections was successfully performed using the developed matrix deposition device. The sensitivity and quality of the images were clearly improved in synchronous mode compared with those of the conventional spray and sublimation methods. These results demonstrate that the integrated device with both solvent nebulization and matrix sublimation is a useful tool in MALDI-MSI applications.
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