4.8 Article

Nanopore Current Enhancements Lack Protein Charge Dependence and Elucidate Maximum Unfolding at Protein's Isoelectric Point

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemical Research Methods

Mapping shifts in nanopore signal to changes in protein and protein-DNA conformation

Autumn Carlsen et al.

Summary: The study investigates the behavior of proteins and DNA in solid-state nanopores and develops a simple geometric model to explain variations in conductance blockage and dwell time observed under different experimental conditions. This research emphasizes the value of nanopore-based protein analysis and represents progress in interpreting complex translocation signals.

PROTEOMICS (2022)

Article Multidisciplinary Sciences

Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis

Ke Xia et al.

Summary: The application of solid-state nanopore devices for analyzing synthetic heparan sulfate GAG chains is feasible, with clear differences between signals generated by different GAGs detected through machine learning and data analysis. These findings suggest that ultrasensitive GAG analysis could be achieved using SS nanopore detection and well-characterized molecular training sets.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Multidisciplinary Sciences

Electrical unfolding of cytochrome c during translocation through a nanopore constriction

Prabhat Tripathi et al.

Summary: The study investigates the electric-field-driven translocation behavior of cytochrome c through ultrathin silicon nitride solid-state nanopores. Results show that protein can pass through narrow pores either by squeezing or by threading after fully unfolding, depending on the pore diameter and unfolding driving force.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Chemistry, Analytical

Discrimination of α-Thrombin and γ-Thrombin Using Aptamer-Functionalized Nanopore Sensing

Lucile Reynaud et al.

Summary: Solid-state nanopores are used for discriminating closely related proteins alpha-thrombin and gamma-thrombin, with aptamer functionalization enhancing discrimination. Postprocessing signals with machine learning and training algorithms, an accuracy of 98.8% is achieved using seven features and ensemble methods.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Analytical

Nanopore Data Analysis: Baseline Construction and Abrupt Change-Based Multilevel Fitting

Y. M. Nuwan D. Y. Bandara et al.

Summary: This study introduces an analysis platform with four baseline fitting methods adaptable to various nanopore traces for extracting events effectively. The performance was tested with stable and fluctuating current profiles, showing that event count increased significantly with increasing fitting robustness for fluctuating profiles. Event turning points were clustered using the dbscan method, with subsequent segmentation and iterative refinement to deduce final event levels.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Multidisciplinary

Modulation of electrophoresis, electroosmosis and diffusion for electrical transport of proteins through a solid-state nanopore

Jugal Saharia et al.

Summary: Nanopore probing of protein transport at the molecular level is greatly influenced by the type of electrolyte, concentration, and solution pH. The study found that different electrolytes led to different protein transport mechanisms, and a new model was developed to better fit the translocation characteristics of proteins, addressing shortcomings of the conventional excluded volume model.

RSC ADVANCES (2021)

Article Nanoscience & Nanotechnology

Calibration-Less DNA Concentration Measurements Using EOF Volumetric Flow and Single Molecule Counting

Nasim Farajpour et al.

Summary: Nanopore sensing is a promising tool for capturing and detecting DNA and other single molecules. Recent research has found that hydrodynamic forces can affect DNA motion against electric field forces, allowing for accurate DNA concentration measurement at low ionic strength conditions. This study provides insights into a unique transport mechanism and proposes a straightforward approach for accurate DNA quantification using volumetric flow.

FRONTIERS IN NANOTECHNOLOGY (2021)

Article Chemistry, Multidisciplinary

Low-Noise Nanopore Enables In-Situ and Label-Free Tracking of a Trigger-Induced DNA Molecular Machine at the Single-Molecular Level

Zhentong Zhu et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Article Chemistry, Multidisciplinary

Adeno-associated virus characterization for cargo discrimination through nanopore responsiveness

Buddini Iroshika Karawdeniya et al.

NANOSCALE (2020)

Article Multidisciplinary Sciences

Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples

Kyloon Chuah et al.

NATURE COMMUNICATIONS (2019)

Article Nanoscience & Nanotechnology

Chemically Functionalizing Controlled Dielectric Breakdown Silicon Nitride Nanopores by Direct Photohydrosilylation

Y. M. Nuwan et al.

ACS APPLIED MATERIALS & INTERFACES (2019)

Article Multidisciplinary Sciences

Surveying silicon nitride nanopores for glycomics and heparin quality assurance

Buddini Iroshika Karawdeniya et al.

NATURE COMMUNICATIONS (2018)

Article Nanoscience & Nanotechnology

Real-time shape approximation and fingerprinting of single proteins using a nanopore

Erik C. Yusko et al.

NATURE NANOTECHNOLOGY (2017)

Article Biophysics

pH Dependence of Charge Multipole Moments in Proteins

Anze Losdorfer Bozic et al.

BIOPHYSICAL JOURNAL (2017)

Article Nanoscience & Nanotechnology

Direct observation of DNA knots using a solid-state nanopore

Calin Plesa et al.

NATURE NANOTECHNOLOGY (2016)

Article Biophysics

High-Bandwidth Protein Analysis Using Solid-State Nanopores

Joseph Larkin et al.

BIOPHYSICAL JOURNAL (2014)

Article Multidisciplinary Sciences

Stiff filamentous virus translocations through solid-state nanopores

Angus McMullen et al.

NATURE COMMUNICATIONS (2014)

Article Multidisciplinary Sciences

A Low-Noise Solid-State Nanopore Platform Based on a Highly Insulating Substrate

Min-Hyun Lee et al.

SCIENTIFIC REPORTS (2014)

Article Chemistry, Multidisciplinary

Fast Translocation of Proteins through Solid State Nanopores

Calin Plesa et al.

NANO LETTERS (2013)

Article Multidisciplinary Sciences

Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field

Kevin J. Freedman et al.

SCIENTIFIC REPORTS (2013)

Article Biochemistry & Molecular Biology

Studying salt effects on protein stability using ribonuclease t1 as a model system

David L. Beauchamp et al.

BIOPHYSICAL CHEMISTRY (2012)

Article Chemistry, Multidisciplinary

Engineering a Rigid Protein Tunnel for Biomolecular Detection

Mohammad M. Mohammad et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

Article Biochemical Research Methods

Integrated nanopore sensing platform with sub-microsecond temporal resolution

Jacob K. Rosenstein et al.

NATURE METHODS (2012)

Article Nanoscience & Nanotechnology

Controlling protein translocation through nanopores with bio-inspired fluid walls

Erik C. Yusko et al.

NATURE NANOTECHNOLOGY (2011)

Article Engineering, Mechanical

Experimental Analysis of Microchannel Entrance Length Characteristics Using Microparticle Image Velocimetry

Tariq Ahmad et al.

JOURNAL OF FLUIDS ENGINEERING-TRANSACTIONS OF THE ASME (2010)

Article Chemistry, Multidisciplinary

Salt dependence of ion transport and DNA translocation through solid-state nanopores

RMM Smeets et al.

NANO LETTERS (2006)