Comparison of Antimicrobial Activity Between Bacitracin-Soaked Sutures and Triclosan Coated Suture

Background: With the easily available option for surgeons to soak their suture in antibiotic irrigation solution intraoperatively in mind, this study was designed to evaluate the ability of suture soaked in bacitracin irrigation solution to inhibit the growth of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. Materials and Methods: Using standard experimental procedure, sterile suture was soaked in Bacitracin, and dried for 10 min or 6 h, incubated for 24 h on inoculated plates, and examined for zone of inhibition around the suture. This was compared to control unsoaked suture and antimicrobial suture (AMS) currently on the market to determine if the minor intraoperative procedural change of placing suture in antibiotic irrigation solution instead of on the sterile table could confer comparable antimicrobial activity. Results: The study found the Bacitracin-soaked suture (BSS) consistently inhibited the growth of the test organisms. For both organisms, the BSS exhibited a significantly larger zone of inhibition compared to the unsoaked control suture ( P < 0.0001). However, both the AMS currently on the market, and a bacitracin aliquot, exhibited significantly larger zones of inhibition compared to both drying times of the BSS ( P < 0.0001). Conclusions: Placing sutures in a bacitracin irrigation solution intraoperatively instead of directly on the sterile table can achieve some of the in vitro antimicrobial effect seen from AMS currently on the market. This may result in reduced rates of surgical site infections and associated costs without major procedural change and at reduced overhead. (c) 2021 Elsevier Inc. All rights reserved.

Automated summary

The study demonstrated that soaking sutures in bacitracin irrigation solution can effectively inhibit the growth of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus, with significantly larger zones of inhibition compared to unsoaked control sutures. While commercially available antimicrobial sutures and bacitracin aliquots showed better inhibition effects, placing sutures in antibiotic irrigation solution during surgery can achieve some in vitro antimicrobial activity.