期刊
JOURNAL OF SURGICAL RESEARCH
卷 267, 期 -, 页码 342-349出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2021.05.044
关键词
Perfluorocarbon emulsions; Platelets; Leukocytes; White cells; Inflammation; Thrombocytopenia; Blood substitute; Artificial blood; Oxygen therapeutic
类别
资金
- Department of the Army - Combat Casualty Care and Materiel Command, Ft. Detrick, Maryland [W81XWH-13-1-0017, PT107803]
The study in sheep model demonstrated minimal impact of PFC infusion on inflammatory cell lines, with a slight decrease in platelet count observed after Oxygent infusion while returning to baseline levels. No platelet effect was seen after Perftoran infusion.
Background: Perfluorocarbon emulsions (PFCs) are intravenous artificial oxygen carriers with enhanced gas solubility. As lipid micelle nanoparticle emulsions, PFCs may have a class effect that causes degrees of thrombocytopenia. Understanding the extent of the platelet effects, including mechanism and potential inflammation after PFC infusion, is important for safe human trials. Methods: Normal sheep (Dorper) were infused with 5 mL/kg of Oxygent (w/v 60% PFC) or Perftoran (w/v 20% PFC). Controls received 6% Hetastarch or were naive. Blood samples were analyzed from baseline, time 0 (the end of infusion), 3 and 24 hours, and 4 and 7 days. Platelet count, plateletcrit, mean platelet volume, platelet distribution width, and CD-62p (a platelet activation-dependent membrane protein) were measured. Neutrophils, monocytes, and total white blood cell counts were analyzed. Results: In these inflammatory cell lines, there were no consistent changes or cellular activation after PFC infusion. A decrease (<10% from baseline and naive controls) in platelet count was seen on day 4 after Oxygent infusion (3 g/kg), which recovered by day 7. No platelet effect was seen in Perftoran (1 g/kg). Plateletcrit, mean platelet volume, and platelet distribution width did not change significantly at any time point among the groups. CD-62p, ADP, and collagen aggregometry showed no significant change in platelet function. Conclusion: There was no evidence of overall reduction in platelet number, or any correlation with the change in platelet activation or inhibition. Therefore, the risk of increased thrombosis/bleeding after PFC intravenous infusion is low in this non-trauma sheep model. (C) 2021 Elsevier Inc. All rights reserved.
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