Core-shell magnetic porous organic polymer for magnetic solid-phase extraction of fluoroquinolone antibiotics in honey samples followed by high-performance liquid chromatography with fluorescence detection

By monomer-mediated in-situ growth synthesis strategy, with hydroquinone and 1,3,5-tris(4-aminophenyl)benzene as monomers, a core-shell magnetic porous organic polymer was synthesized through a simple azo reaction. Based on this, a magnetic solid-phase extraction-high-performance liquid chromatography-fluorescence detection method was proposed for the analysis of fluoroquinolones in a honey sample. With ofloxacin, ciprofloxacin, enrofloxacin, lomefloxacin, and difloxacin as target analytes, factors affecting the extraction efficiency had been optimized. The LODs were 1.5-5.4 ng/L (corresponding to 0.23-0.81 mu g/kg in honey). The linear range was 0.005-20 mu g/L for difloxacin, 0.01-20 mu g/L for ofloxacin, ciprofloxacin and lomefloxacin, and 0.02-20 mu g/L for enrofloxacin. The enrichment factor was 84.4-91.7-fold with a high extraction efficiency of 84.4-91.7%. The method was assessed by the analysis of target fluoroquinolones in honey samples, and the recoveries for the spiked samples were 79.3-95.8%. The results indicated that the established magnetic solid-phase extraction-high-performance liquid chromatography-fluorescence detection method is efficient for the analysis of trace fluoroquinolones in honey.

Automated summary

A core-shell magnetic porous organic polymer was synthesized through a monomer-mediated in-situ growth synthesis strategy, enabling the development of a magnetic solid-phase extraction-high-performance liquid chromatography-fluorescence detection method for the analysis of fluoroquinolones in honey samples. The method showed high extraction efficiency and recoveries, with optimized linear ranges and limits of detection for the target analytes in honey.