期刊
JOURNAL OF SEPARATION SCIENCE
卷 45, 期 9, 页码 1570-1579出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100900
关键词
diterpenoids; high-speed countercurrent chromatography; preparative high-performance liquid chromatography; traditional Chinese medicine
资金
- Key-Area Research and Development Program of Guangdong Province [2020B1111110003]
- Guilin Innovation Platform and Talent Plan [20210102-3]
- Guangxi Science and Technology Base and Talent Project [AA21196009]
- West Light Foundation of the Chinese Academy of Sciences [[2018] 6]
- Foundation of Guangxi Key Laboratory of Functional Phytochemicals Research and Utilisation [ZRJJ2019-5]
In this study, an efficient strategy using high-speed countercurrent chromatography combined with preparative high-performance liquid chromatography was developed to separate diterpenoid compounds from complex crude extracts of Salvia bowleyana Dunn roots. The results demonstrated that this method can effectively isolate diterpenoids, including minor components, from complex natural products.
The root of Salvia bowleyana Dunn (Lamiaceae) is used as a traditional Chinese medicine that has multiple therapeutic effects. In this study, an efficient strategy was developed to separate diterpenoid compounds, which are the main active ingredients in Salvia bowleyana Dunn roots, from complex crude extracts by high-speed countercurrent chromatography combined with preparative high-performance liquid chromatography. A two-phase solvent system comprising n-hexane-ethyl acetate-methanol-water (7:3:7:3, v/v/v/v) was selected for high-speed countercurrent chromatographic separation. Three major diterpenoids, 6 alpha-hydroxysugiol (7), sugiol (8), and 6, 12-dihydroxyabieta-5,8,11,13-tetraen-7-one (9) were obtained at purities of 98.9, 95.4, and 96.2%, respectively, and minor diterpenoids were enriched via one-step separation. The enriched minor diterpenoids were further purified by continuous preparative high-performance liquid chromatography to yield two new norabietanoids (1, 6) and four known compounds (2-5). The structures of these new compounds were determined using NMR spectroscopy, high-resolution electrospray ionization mass spectrometry, and electronic circular dichroism spectroscopy. The results suggest that high-speed countercurrent chromatography combined with preparative high-performance liquid chromatography efficiently isolates diterpenoids, including minor components, from complex natural products.
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