期刊
JOURNAL OF SEPARATION SCIENCE
卷 45, 期 6, 页码 1195-1201出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100835
关键词
chiral separation; cyclodextrins; open-tubular liquid chromatography; semipermanent coating; stationary pseudophase coating
资金
- Australian Research Council [DP180102810, 2018]
This study demonstrates the successful chiral separation of various analytes using different cyclodextrins as mobile phase additives in open-tubular liquid chromatography with a stable coating. The method achieved high resolutions and low %RSD values.
The chiral separation of various analytes (dichlorprop, mecoprop, ibuprofen, and ketoprofen) was demonstrated with different cyclodextrins as mobile phase additives in open-tubular liquid chromatography using a stationary pseudophase semipermanent coating. The stable coating was prepared by a successive multiple ionic layer approach using poly(diallyldimethylammonium chloride), polystyrene sulfonate, and didodecyldimethyl ammonium bromide. Increasing concentrations (0-0.2 mM) of various native and derivatized cyclodextrins in 25 mM sodium tetraborate (pH 9.2) were investigated. Chiral separation was achieved for the four test analytes using 0.05-0.1 mM beta-cyclodextrin (resolution between 1.11 and 1.34), gamma-cyclodextrin (resolution between 0.78 and 1.27), carboxymethyl-beta-cyclodextrin (resolution between 1.64 and 2.59), and 2-hydroxypropyl-beta-cyclodextrin (resolution between 0.71 and 1.76) with the highest resolutions obtained with 0.1 mM carboxymethyl-beta-cyclodextrin. %RSD values were <10%. This is the first demonstration of chiral open-tubular liquid chromatography using achiral chromatographic coatings and cyclodextrins as mobile phase additives.
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