In vivo and in vitro evaluation of the wound healing properties of chitosan extracted from Trametes versicolor

Nanofiber dressings, a group of bioactive wound dressings, have received a great deal of attention due to their unique properties, such as surface-to-volume ratio, three-dimensional structure, and porosity. These wound dressings accelerate wound healing by maintaining moisture at the injury site, preventing infection, and increasing oxygen delivery. Due to its high compatibility, biodegradability, and bioactivity, chitosan is a widely used compound in wound dressings. According to studies, one of the best sources for chitosan is its extraction and production from medicinal fungi. Mycelium of the fungus Trametes versicolor is used to produce chitosan. In this study, nanofibers containing T. versicolor chitosan and PVA were prepared by electrospinning, and their wound healing properties were investigated. The manufacturing conditions for nanofibers were optimized, and their properties were investigated using various analytical techniques, including SEM, FTIR, and tensile mechanical testing. Nanofibers' biological properties (antibacterial and cytotoxicity) and their effects on wound healing were investigated. T. versicolor chitosan/PVA nanofibers with a ratio of 25:75, a feed rate of 0.6 mL/min, and a voltage of 20 kV were prepared. SEM images showed that the nanofibers were 276 nm in diameter; the nanofibers were uniform and had no beads. Bacterial growth inhibition for T. versicolor chitosan/PVA nanofibers was 57.5 % and 93 % against E. coli and S. aureus, respectively. Animal experiments evaluating the efficacy of nanofibers revealed that T. versicolor chitosan/PVA nanofibers had wound healing rate of 95 %. T. versicolor chitosan/PVA nanofibers allow fibroblast cells to adhere and grow by facilitating the exchange of moisture and oxygen and accelerating the wound healing process. T. versicolor chitosan/PVA nanofibers have good potential for use as wound dressings.

Automated summary

Nanofiber dressings incorporating chitosan and other compounds accelerate wound healing with potential for various applications, exhibiting inhibitory effects on bacterial growth.