期刊
IMMUNOLOGY
卷 149, 期 2, 页码 157-171出版社
WILEY
DOI: 10.1111/imm.12630
关键词
antigen transfer; dead-cell-associated antigen; dendritic cells; exosomes; macrophages
类别
资金
- National Natural Science Foundation of China [81302603, 81471553, 81172882, 81272315, 81172791, 81202308, 81273212]
- National Basic Research Programme of China [2015CB943203]
- National Science and Technology Major Projects [2014ZX 09508003]
- National Science Foundation of Shandong Provice, China [ZR2011CM037, J13LK01]
Defects in rapid clearance of apoptotic cells lead to an accumulation of dead cells (late apoptotic or secondary necrotic cells), which results in an aberrant immune response. However, little is known about whether and how macrophages (M phi s) cooperate with dendritic cells (DCs) in the presentation of dead-cell-associated antigens in this process. By transferring high numbers of dead cells to mimic a failure of apoptotic cell clearance in vivo, we found that M phi s and neutrophils were the predominant phagocytes in the uptake of dead cells in the spleen. Moreover, both M phi s and DCs were required for an optimal CD4(+) T-cell response triggered by dead-cell-associated antigens. Importantly, although M phi s alone had a poor capacity for antigen presentation, they could transfer phagocytosed antigens to DCs for potent antigen presentation to enhance T-cell responses. Finally, we found that exosomes released from M phi s acted as a transmitter to convey antigens to DCs partially in a ceramide-dependent manner, since treatment with the neutral sphingomyelinase inhibitor GW4869 and spiroepoxide resulted in a significant reduction of T-cell proliferation in vitro and in vivo. These findings point to a novel pathway of cross-talk between M phi s and DCs, which will be helpful to explain possible mechanisms for autoimmune diseases characterized by increased rates of apoptosis.
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