期刊
IMMUNOBIOLOGY
卷 221, 期 11, 页码 1237-1246出版社
ELSEVIER GMBH
DOI: 10.1016/j.imbio.2016.06.010
关键词
Macrophage; Monocyte; M1; M2; Macrophage polarisation; Biomaterials; Surface chemistry; Oxygen plasma etching; Water contact angle; Foreign body response
类别
资金
- EU FP7 IMMODGEL [602694]
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/N006615/1]
- Royal Society
- Human Capacity Development Program (HCDP) PhD scholarship (Kurdistan Regional Government)
- EPSRC [EP/N006615/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/N006615/1] Funding Source: researchfish
Macrophages are innate immune cells that have a central role in combating infection and maintaining tissue homeostasis. They exhibit remarkable plasticity in response to environmental cues. At either end of a broad activation spectrum are pro-inflammatory (Ml) and anti-inflammatory (M2) macrophages with distinct functional and phenotypical characteristics. Macrophages also play a crucial role in orchestrating immune responses to biomaterials used in the fabrication of implantable devices and drug delivery systems. To assess the impact of different surface chemistries on macrophage polarisation, human monocytes were cultured for 6 days on untreated hydrophobic polystyrene (PS) and hydrophilic O-2 plasma-etched polystyrene (O-2-PS40) surfaces. Our data clearly show that monocytes cultured on the hydrophilic O-2-PS40 surface are polarised towards an Ml-like phenotype, as evidenced by significantly higher expression of the pro-inflammatory transcription factors STAT1 and IRF5. By comparison, monocytes cultured on the hydrophobic PS surface exhibited an M2-like phenotype with high expression of mannose receptor (MR) and production of the anti-inflammatory cytokines IL-10 and CCL18. While the molecular basis of such different patterns of cell differentiation is yet to be fully elucidated, we hypothesise that it is due to the adsorption of different biomolecules on these surface chemistries. Indeed our surface characterisation data show quantitative and qualitative differences between the protein layers on the O-2-PS40 surface compared to PS surface which could be responsible for the observed differential macrophage polarisation on each surface. (C) 2016 The Authors. Published by Elsevier GmbH.
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