期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 226, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2021.112369
关键词
Myeloperoxidase; Pulse radiolysis; Chlorinating activity; Oxidative stress; Inflammation
资金
- Spanish Ministerio de Ciencia y Tecnologia [CTQ2004-00534/BQU]
- European Commission [41365]
- regional government of the Xunta de Galicia [GPC ED431B 2020/52]
MPO is an enzyme mainly expressed in the azurophilic granules of neutrophils, and it plays a role in the defense against pathogens by catalyzing the formation of reactive oxygen species. Research shows that ionizing radiation affects MPO activity at peripheral functional groups rather than at its core structure. These findings are important for understanding the resistance mechanism of our innate anti-pathogenic defense system and for developing therapeutic strategies for autoimmune diseases.
Myeloperoxidase (MPO) is a myeloid-lineage restricted enzyme largely expressed in the azurophilic granules of neutrophils. It catalyses the formation of reactive oxygen species, mainly hypochlorous acid, contributing to anti-pathogenic defense. Disorders in the production or regulation of MPO may lead to a variety of health conditions, mainly of inflammatory origin, including autoimmune inflammation.We have studied the effect of ionizing radiation on the activity of MPO, as measured by the capacity retained by the enzyme to produce hypochlorous acid as reactive oxygen species after exposure to successive doses of solvated electrons, the strongest possible one-e(-) reducing agent in water. Chlorination activity was still present after a very high irradiation dose, indicating that radiation damage does not take place at the active site, hindered in the core of MPO structure. Decay kinetics show a dependence on the wavelength, supporting that the process must occur at peripheral functional groups situated on external and readily accessible locations of the enzyme.These results are relevant to understand the mechanism of resistance of our innate anti-pathogenic defense system and also to get insight into potential strategies to regulate MPO levels as a therapeutic target in autoimmune diseases.
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