期刊
JOURNAL OF PEST SCIENCE
卷 96, 期 1, 页码 181-193出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10340-022-01485-5
关键词
HpRNA expression system; Nanocarrier; Nanocarrier; hpRNA pesticide; RNA interference; RNA pesticide
类别
In this study, a nanocarrier-based transdermal dsRNA delivery system and a bacteria-based hairpin RNA (hpRNA) expression system were used to develop a sprayable RNA pesticide. The greenhouse trial on green peach aphid showed that the RNA pesticide had a certain insecticidal activity, with a total control efficacy of 61% on day 3 and maintained at 50% until the sixth day. This study is the first attempt to apply bacteria-expressed and nanocarrier-delivered RNA pesticides for pest control in a greenhouse, which is beneficial for promoting the development of RNA pesticides.
There are two main limitations for sprayable RNA pesticide development: delivery efficiency and synthetic cost of double-stranded RNA (dsRNA). We previously constructed a nanocarrier-based transdermal dsRNA delivery system and a novel bacteria-based hairpin RNA (hpRNA) expression system to solve these challenges. Herein, as a subsequent exploration of RNA pesticide (sprayable ds/hpRNA for pest control), we performed a greenhouse application of bacteria-expressed and nanocarrier-delivered RNA pesticide on green peach aphid. The nanoparticle SPc could combine and deliver dsRNA across the aphid cuticle and V-type proton ATPase subunits d (ATP-d) and G (ATP-G) were selected as the potential RNA interference (RNAi) targets. Our plasmid-Escherichia coli system simultaneously expressing ATP-d and ATP-G hairpin RNAs (hpRNAs) was constructed for mass production of hpRNA. The expressed hpRNA was mixed with SPc and detergent to form RNA formulation, which showed a certain insecticidal activity through the spray application in the greenhouse. Total control efficacy of our RNA pesticide could reach 61% on 3 d and maintained at 50% until the sixth day. To our knowledge, our study is the first attempt to apply the bacteria-expressed and nanocarrier-delivered RNA pesticides for pest control in the greenhouse trial, which is beneficial for promoting the development of RNA pesticides.
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