期刊
IMMUNOBIOLOGY
卷 221, 期 9, 页码 944-952出版社
ELSEVIER GMBH
DOI: 10.1016/j.imbio.2016.05.011
关键词
Complement; Factor H; Mycobacterium; Phagocytosis; Macrophage; Tuberculosis; Cytokine
类别
资金
- Ministry of Higher Education, Malaysia
- Universiti Sains Malaysia
Mycobacterium tuberculosis is an accomplished intracellular pathogen, particularly within the macrophage and this is of the utmost importance in the host-pathogen stand-off observed in the granuloma during latent tuberculosis. Contact with innate immune molecules is one of the primary interactions that can occur with the pathogen M. tuberculosis once inhaled. Complement proteins may play a role in facilitating M. tuberculosis interactions with macrophages. Here, we demonstrate that factor H, a complement regulatory protein that down-regulates complement alternative pathway activation, binds directly to the model organism M. bovis BCG. Binding of factor H reaches saturation at 5-10 mu g of factor H/ml, well below the plasma level. C4 binding protein (C4BP) competed with factor H for binding to mycobacteria. Factor H was also found to inhibit uptake of M. bovis BCG by THP-1 macrophage cells in a dose-dependent manner. Real-time qPCR analysis showed stark differential responses of pro- and anti-inflammatory cytokines during the early stages of phagocytosis, as evident from elevated levels of TNF-alpha., IL-1 beta and IL-6, and a concomitant decrease in IL-10, TGF-beta and IL-12 levels, when THP-1:BCG interaction took place in the presence of factor H. Our results suggest that factor H can interfere with mycobacterial entry into macrophages and modulate inflammatory cytokine responses, particularly during the initial stages of infection, thus affecting the extracellular survival of the pathogen. Our results offer novel insights into complement activation-independent functions of factor H during the host-pathogen interaction in tuberculosis. (C) 2016 The Author(s). Published by Elsevier GmbH.
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