期刊
JOURNAL OF PAIN
卷 23, 期 4, 页码 547-557出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.jpain.2021.10.002
关键词
Morphine; neuropathic pain; serotonin; descending pain modulation; gamma-aminobutyric acid
资金
- Ministry of Education, Culture, Sports, Scientific and Technology of Japan [18K16437, 26670678, 25670664]
This study demonstrates that dysfunction of the descending serotonergic system and functional changes in GABA A receptors contribute to the attenuated efficacy of morphine against neuropathic pain. Activation of spinal 5-HT3 receptors increases GABA concentration, and morphine activates GABAergic interneurons in the spinal dorsal horn through descending serotonergic neurons.
Systemic administration of morphine increases serotonin (5-HT) in the spinal dorsal horn (SDH), which attenuates the analgesic effects of morphine on neuropathic pain through spinal 5-HT3 receptors. We hypothesized that dysfunction of the descending serotonergic system, including the periaqueductal gray (PAG), contributes to attenuate the efficacy of morphine on neuropathic pain through spinal 5-HT3 receptors and GABA neurons. Morphine (100 ng) injected into the PAG produced analgesic effects in normal rats, but not in spinal nerve ligation (SNL) rats. In vivo microdialysis showed that PAG morphine increased the SDH 5-HT concentration in both groups. Intrathecal injection of the 5-HT3 receptor antagonist ondansetron and the GABA A receptor antagonist bicuculline attenuated the analgesic effects of PAG morphine in normal rats, but increased the effects in SNL rats. The increased analgesic effect of PAG morphine induced by bicuculline was reversed by pretreatment with the tropomyosin receptor kinase B (TrkB) antagonist K252a. Activation of spinal 5-HT3 receptors by 2-methyl-5-HT increased the GABA concentration in both groups. Morphine activates GABAergic interneurons in the SDH by activating descending serotonergic neurons. Functional changes in GABA A receptors from inhibitory to facilitatory through the activation of TrkB receptors may contribute to the attenuated efficacy of morphine against neuropathic pain. Perspective: Although morphine provides strong analgesia against acute pain, it has limited efficacy against neuropathic pain. This article demonstrates that functional changes in GABA A receptors in the spinal dorsal horn after nerve injury might strongly contribute to the attenuation of opioid-induced analgesia for neuropathic pain. (C) 2021 The Author(s). Published by Elsevier Inc. on behalf of United States Association for the Study of Pain, Inc.
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