期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 87, 期 1, 页码 137-146出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.joc.1c02056
关键词
-
资金
- Academy of Finland [286478]
- Academy of Finland (AKA) [286478, 286478] Funding Source: Academy of Finland (AKA)
A novel organomercury nucleobase analogue was synthesized and incorporated into an oligonucleotide, followed by mercuration. The affinity of the organomercury nucleobase analogue towards complementary strands was studied using UV, CD, and F-19 NMR spectroscopy, revealing distinct affinities for different canonical nucleobases. Results showed that the F-19 resonance signal was sensitive to local environmental changes, providing valuable information on the binding of the mercurated oligonucleotide to complementary strands at varying temperatures.
A 2-trifluoromethylaniline C-nucleoside was synthesized, incorporated in the middle of an oligonucleotide, and mercurated. The affinity of the mercurated oligonucleotide toward complementary strands placing each of the canonical nucleobases opposite to the organomercury nucleobase analogue was examined by ultraviolet (UV), circular dichroism (CD), and F-19 NMR spectroscopy analyses. According to the UV melting profile analysis, the organomercury nucleobase analogue showed increased affinities in the order T > G > C > A. The CD profiles indicated the typical B-type helix in each case. The F-19 resonance signal proved sensitive for the local environmental changes, showing clearly distinct signals for the duplexes with different opposing nucleobases. Furthermore, valuable information on the mercurated oligonucleotide and its binding to complementary strands at varying temperature could be obtained by F-19 NMR spectroscopy.
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