期刊
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
卷 48, 期 2, 页码 420-430出版社
WILEY
DOI: 10.1111/jog.15122
关键词
female; infertility; nutrition; ovarian function; pharmacology; postpartum care
资金
- National Natural Science Foundation of China [81770527, 82171700]
- Natural Science Foundation of Guangdong [2017A020214020]
- Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi [2020KY12033]
SBH with M-n below 4000 Da was prepared through heating extraction, enzymatic hydrolysis, and ultrafiltration. Moderate (400 mg/kg) SBH dose showed the greatest effect on remission of ovarian injury in mice, improving estrus cycle and follicle numbers.
Aims This study aimed to prepare swim bladder hydrolysate (SBH) with M-n < 4000 Da, and investigate its effects on cyclophosphamide (CTX)-mediated ovarian injury in mice. Methods Hydrolysates were prepared by heating extraction, enzymatic hydrolysis and ultrafiltration. M-n and distribution of SBH were analyzed via gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Changes in the mouse oestrus cycle were determined by cytological examination. The number of follicles was examined using histopathology. Enzyme-linked immunosorbent assays (ELISAs) were used to determine the serum sex hormone levels. Results The M-n of SBH, prepared by heating extraction, enzymatic hydrolysis, ultrafiltration, and from different batches, was below 4000 Da, and the preparation process was stable. Compared with the control group, the low-, middle-, and high-dose SBH treatment groups showed different trends in oestrus duration, serum sex hormone levels, and the number of primordial and secondary follicles. The oestrus cycle duration of the high-dose SBH group was longer than that of the model group. The serum luteinizing hormone, follicle-stimulating hormone, and anti-Mullerian hormone levels in the middle-dose group were the closest to those of control group. The number of primordial and secondary follicles in the medium-dose group was significantly higher than that in the model group and closest to those of control group. Conclusion After heating extraction, trypsin/Flavourzyme hydrolysis and ultrafiltration, a hydrolysate with M-n below 4000 Da could be prepared. We found that a moderate (400 mg/kg) SBH dose resulted in the greatest effect on ovarian injury remission in mice.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据