Biotin-functionalized silica nanoparticles loaded with Erythrosine B as selective photodynamic treatment for Glioblastoma Multiforme

In this contribution, we report the development of a biotin-functionalized silica nanoparticle loaded with Erythrosine B (ERY) to act as a selective cancer nanoplatform via Photodynamic Therapy. Amino silica nanoparticles were synthesized by the co-condensation method and further functionalized via amide linkage with biotin. The silica nanoparticles (mean diameter similar to 120 nm) were loaded with ERY yieldings of 34.9 mg g(-1) and 16.7 mg g(-1), respectively, for the non-biotinylated (ERY/SiNP) and biotinylated (ERY/SiNP-B). The adsorption followed two consecutive first-order reactions, and the isotherms fitted to the Langmuir (SiNP) and Freundlich (SiNP-B) models. The ERY is strongly attached to the silica nanoparticle, which prevents their premature leakage to the biological fluids. The well-established effect of the uric acid as a singlet oxygen scavenger was used to show the O-1(2) formation by the nanoplatforms. Trypan blue and MTT cytotoxic assays have shown that ERY/SiNP and ERY/SiNP-B samples are potential systems for PDT applications against Gliobastoma Multiforme (GBM) cancer cells vanishing around 50 % of T98G cells. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study developed a cancer nanoplatform for Photodynamic Therapy by loading Erythrosine B onto silica nanoparticles, showing selective effectiveness against Gliobastoma Multiforme (GBM) cancer cells. The nanoparticles exhibited strong attachment of Erythrosine B, preventing premature leakage, and potential applications for PDT with around 50% cell elimination.