Genetic Code Expansion Through Quadruplet Codon Decoding

Noncanonical amino acid mutagenesis has emerged as a powerful tool for the study of protein structure and function. While triplet nonsense codons, especially the amber codon, have been widely employed, quadruplet codons have attracted attention for the potential of creating additional blank codons for noncanonical amino acids mutagenesis. In this review, we discuss methodologies and applications of quadruplet codon decoding in genetic code expansion both in vitro and in vivo. (C) 2021 Elsevier Ltd. All rights reserved.

Automated summary

Noncanonical amino acid mutagenesis is a powerful tool for studying protein structure and function. Quadruplet codons have attracted attention for creating additional blank codons in noncanonical amino acid mutagenesis. This review discusses the methodologies and applications of quadruplet codon decoding in genetic code expansion both in vitro and in vivo.