期刊
JOURNAL OF MICROBIOLOGY
卷 60, 期 2, 页码 215-223出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-022-1649-3
关键词
Bacteroides thetaiotaomicron; hybrid two-component system; mucin; colonization; colitis
类别
资金
- Suh Kyungbae Foundation
- POSCO Science Fellowship of POSCO TJ Park Foundation
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Republic of Korea [2020R1I1A1A-01052942]
- BK21 Fellowship from the Ministry of Education
The study identifies a genetic determinant, BT2391, for Bacteroides thetaiotaomicron colonization in the gut. The BT2391 mutant shows increased competitive fitness and adherence ability, but it may promote colitis under specific dietary conditions.
The mammalian intestinal tract contains trillions of bacteria. However, the genetic factors that allow gut symbiotic bacteria to occupy intestinal niches remain poorly understood. Here, we identified genetic determinants required for Bacteroides thetaiotaomicron colonization in the gut using transposon sequencing analysis. Transposon insertion in BT2391, which encodes a hybrid two-component system, increased the competitive fitness of B. thetaiotaomicron. The BT2391 mutant showed a growth advantage in a mucin-dependent manner and had an increased ability to adhere to mucus-producing cell lines. The increased competitive advantage of the BT2391 mutant was dependent on the BT2392-2395 locus containing susCD homologs. Deletion of BT2391 led to changes in the expression levels of B. thetaiotaomicron genes during gut colonization. However, colonization of the BT2391 mutant promoted DSS colitis in low-fiber diet-fed mice. These results indicate that BT2391 contributes to a sustainable symbiotic relationship by maintaining a balance between mucosal colonization and gut homeostasis.
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