期刊
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷 44, 期 11-12, 页码 610-617出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2021.2007947
关键词
Fingerprint; principal component analysis; quality control; Rheum palmatum; rhubarb
资金
- National Key RD Program [2019YFC1711500]
- Nature Science Foundation of Jiangsu Higher Education Institutions of China [19KJA180008]
- Jiangsu Provincial Innovation and Entrepreneurship Talent Plan [202010528]
The study established a UPLC fingerprint method for quality evaluation of Rheum palmatum and comparison with other related species. The results showed that the fingerprint profiles could distinguish Rheum palmatum from different original plants and confusable varieties. Principal component analysis indicated discrepancies in chemical components among three different species of rhubarb.
Rheum palmatum is one of the three genuine Rhubarb in Chinese Pharmacopeia and widely used in clinic as a purgative medicine. To effective quality evaluation of R. palmatum, a UPLC fingerprint method was established. Twenty batches of R. palmatum were analyzed, together with R. tanguticum, R. officinale and Polygonum cuspidatum. Fourteen common peaks were detected and four of them were identified by comparing with reference substances. The similarity degrees of 20 batches of R. palmatum were between 0.94 and 1.00. Rhubarbs from different original plants, along with confusable variety P. cuspidatum, can be readily distinguished through their fingerprint profiles. Principal component analysis was performed by using SIMCA 14.1 to compare three different rhubarbs. The results showed that three species of rhubarb can be distinguished well, indicating their discrepancies in chemical components. The established UPLC fingerprint is stable and reliable, which can be used for quality evaluation of R. palmatum, as well as comparison of rhubarbs from different original plants and its confusable variety. This study provides theoretical guidance for comprehensively improving the quality control level of R. palmatum.
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