HPLC determination of pKa value of benfotiamine

Physicochemical characteristics such as the dissociation constant (pKa) and lipophilicity parameter are very important for the properties of compounds and their determination is of great interest to the research field. They affect the solubility and permeability of the active pharmaceutical ingredients and respectively the penetration through the cellular membrane. The higher value of the lipophilicity parameter contributes to the better interaction of the investigated substances with the cellular membrane and boosts their biological action. Utilizing HPLC methods for the determination of such parameters helps to overlap challenges such as poor solubility and purity of the investigated substances. An analytical HPLC method for the determination of the pKa values and the lipophilicity parameter of benfotiamine is used in the current work.

Automated summary

Physicochemical characteristics such as pKa and lipophilicity parameter play a crucial role in determining the properties and biological activity of compounds. Utilizing HPLC methods can help overcome challenges related to solubility and purity of investigated substances. High lipophilicity parameter contributes to enhanced biological action through better interaction with cellular membranes.