期刊
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
卷 64, 期 1, 页码 183-201出版社
WILEY
DOI: 10.1111/jipb.13194
关键词
apoplastic effector; elicitor; Nicotiana benthamiana; oomycete; receptor-like kinase
资金
- Fundamental Research Funds for the Central Universities [KYLH201703]
- National Natural Science Foundation of China [32072507]
The PcEXLX1 protein from Nicotiana benthamiana was found to activate plant immune responses and enhance resistance against Phytophthora capsici. The perception of PcEXLX1 was regulated by the receptor-like kinase ERK1.
Phytophthora capsici is one of the most harmful pathogens in agriculture, which threatens the safe production of multiple crops and causes serious economic losses worldwide. Here, we identified a P. capsici expansin-like protein, PcEXLX1, by liquid chromatography-tandem mass spectrometry from Nicotiana benthamiana apoplastic fluid infected with P. capsici. Clustered regularly interspaced short palindromic repeats/crispr associated protein 9 (CRISPR/Cas9)-mediated PcEXLX1 knockout mutants exhibited significantly enhanced virulence, while the overexpression of PcEXLX1 impaired the virulence. Prokaryotically expressed PcEXLX1 activated multiple plant immune responses, which were BRI1-associated kinase 1 (BAK1)- and suppressor of BIR1-1 (SOBIR1)-dependent. Furthermore, overexpression of PcEXLX1 homologs in N. benthamiana could also increase plant resistance to P. capsici. A G-type lectin receptor-like kinase from N. benthamiana, expansin-regulating kinase 1 (ERK1), was shown to regulate the perception of PcEXLX1 and positively mediate the plant resistance to P. capsici. These results reveal that the expansin-like protein, PcEXLX1, is a novel apoplastic effector with plant immunity-inducing activity of oomycetes, perception of which is regulated by the receptor-like kinase, ERK1.
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