期刊
JOURNAL OF INFECTIOUS DISEASES
卷 226, 期 2, 页码 208-216出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiac005
关键词
COVID-19; SARS-CoV-2; Cytokine release assay; T cells; nucleocapsid; spike
资金
- Swedish Research Council [2020-01437]
- AFA Insurance Foundation [20045]
- Clas Groschinsky Foundation
- Wilhelm and Martina Lundgren Research Foundation [2021-3819]
- Sahlgrenska Academy at the University of Gothenburg
- Swedish Research Council [2020-01437] Funding Source: Swedish Research Council
A whole-blood stimulation assay was developed to detect SARS-CoV-2-specific T cells in previously infected or vaccinated individuals. The study found that T-cell responses persisted longer than immunoglobulin G responses and could be used to evaluate complications and vaccine-induced T-cell immunity.
We developed a simple and sensitive whole-blood stimulation assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cells in previously infected or vaccinated individuals. Virus-specific T-cell responses persisted significantly longer than SARS-CoV-2-specific immunoglobulin G responses. Background Waning of immunoglobulin G (IgG) antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) complicates the diagnosis of past infection. The durability of T-cell memory against SARS-CoV-2 remains unclear, and most current T-cell protocols are unsuited for large-scale automation. Methods Whole-blood samples from 31 patients with verified past coronavirus disease 2019 (COVID-19) and 46 controls, of whom 40 received COVID-19 vaccine, were stimulated with peptides spanning the nucleocapsid (NC) or spike 1 (S1) regions of SARS-CoV-2 and analyzed for interferon gamma in supernatant plasma. Diagnostic accuracy of these assays was evaluated against serum anti-NC and anti-receptor-binding domain S1-IgG. Results Induction of interferon gamma in whole blood by NC or S1 peptides diagnosed past COVID-19 with high accuracy (area under the receiver operating characteristic curve, 0.93 and 0.95, respectively). In accordance with previous studies, NC-IgG levels rapidly waned with only 5 of 17 patients (29%) remaining seropositive >180 days after infection. By contrast, NC peptide-induced T-cell memory responses remained in 13 of 17 study participants (76%) >180 days after infection (P = .01 for comparison with NC-IgG; McNemar test). After 2 vaccine doses, all 18 donors exhibited S1-specific T-cell memory. Conclusions Cytokine release assays for the monitoring of T-cell memory in whole blood may be useful for evaluating complications following unverified past COVID-19 and for long-term assessment of vaccine-induced T-cell immunity.
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