Ethanol production by Escherichia coli from detoxified lignocellulosic teak wood hydrolysates with high concentration of phenolic compounds

Teak wood residues were subjected to thermochemical pretreatment, enzymatic saccharification, and detoxification to obtain syrups with a high concentration of fermentable sugars for ethanol production with the ethanologenic Escherichia coli strain MS04. Teak is a hardwood, and thus a robust deconstructive pretreatment was applied followed by enzymatic saccharification. The resulting syrup contained 60 g l(-1) glucose, 18 g l(-1) xylose, 6 g l(-1) acetate, less than 0.1 g l(-1) of total furans, and 12 g l(-1) of soluble phenolic compounds (SPCs). This concentration of SPC is toxic to E. coli, and thus two detoxification strategies were assayed: (1) treatment with Coriolopsis gallica laccase followed by addition of activated carbon and (2) overliming with Ca(OH)(2). These reduced the phenolic compounds by 40% and 76%, respectively. The detoxified syrups were centrifuged and fermented with E. coli MS04. Cultivation with the overlimed hydrolysate showed a 60% higher volumetric productivity (0.45 g(ETOH) l(-1) hr(-1)). The bioethanol/sugar yield was over 90% in both strategies.

Automated summary

In this study, teak wood residues were pretreated, enzymatically saccharified, and detoxified to obtain syrups with high concentrations of fermentable sugars for ethanol production. Two detoxification strategies were tested, which successfully reduced the toxicity of phenolic compounds and increased the ethanol productivity. Over 90% ethanol/sugar yield was achieved in both strategies.