Enhanced Pb(II) removal from water using conductive carbonaceous nanomaterials as bacterial scaffolds: An experimental and modelling approach

This study investigated the abilities of electrochemically active bacteria (EAB) as bio-catalysts for Pb(II) bioreduction in an attached growth reactor. Electrically conductive carbon nanofibers (CNF) were used as bacterial scaffolds to facilitate electron transfer between EAB and Pb(II). Our results demonstrated that the CNF attached growth reactor can remove > 80% and > 90% of Pb(II) from solution at 0.5 mg/L and 5.0 mg/L concentrations, respectively, and maintained Pb(II) levels < 0.10 mg/L over repeated Pb(II) dosages dosed at all concentrations. Experimental measurements by SEM-EDX and ICP-OES provided evidence that Pb(II) was reduced to Pb-0, suggesting that Pb(II) was adsorbed onto CNFs, and subsequently bioreduced. Numerical modelling was used to estimate Pb(II) concentrations along the CNFs via capturing Pb(II)'s diffusive transport, and the removal mechanisms governed by EAB and CNF adsorption. The model simulation results demonstrated that Pb(II) bioreduction was dominant in the reactor and the significance of Monod kinetics, biofilm thickness and Pb(II) dosage concentration on its removal. This study is the first to prove EAB's ability to treat Pb(II) from contaminated waters in an attached growth configuration. The findings here demonstrate that CNFs can boost biological treatment efficacies in wastewater and/or water treatment to meet stringent water guidelines.

Automated summary

This study investigates the abilities of electrochemically active bacteria (EAB) to bioreduce Pb(II) in an attached growth reactor. The results show that a reactor with electrically conductive carbon nanofibers (CNFs) as bacterial scaffolds can efficiently remove Pb(II) from solution at different concentrations. Experimental measurements and numerical modeling provide evidence that suggests the adsorption of Pb(II) onto CNFs and subsequent bioreduction. This study is the first to demonstrate EAB's ability to treat Pb(II) in contaminated waters using an attached growth configuration, highlighting the potential of CNFs in boosting biological treatment efficacy.