期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 423, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.127058
关键词
cyanazine; herbicide; DNA biosensor; monitoring; hazardous substance
The research focused on monitoring cyanazine levels using an electrochemical strategy, synthesizing a Pt-Pd-CdO/SWCNTs composite and modifying a gold electrode for detection. The composite showed improved conductivity and lower charge-transfer resistance, allowing for monitoring of cyanazine at nanomolar concentrations. Differential pulse voltammograms indicated a linear dynamic range and detection limit, while molecular docking studies supported experimental results. The composite was successfully utilized for monitoring cyanazine in food and water samples.
Cyanazine is a beneficial herbicide in the triazines group that inhibits photosynthesis in plants and monitoring of this herbicide is so important for study agriculture products. The present researches have been focused on monitoring of cyanazine by a straightforward and fast electrochemical strategy. Herein, to monitor the cyanazine level, Pt and Pd doped CdO nanoparticle decorated SWCNTs composite (Pt-Pd-CdO/SWCNTs) has been synthesized as a conductive mediator and characterized by EDS, SEM and TEM techniques. The Pt-Pd-CdO/SWCNTs and ds-DNA have been used for modification of the gold electrode (GE). Moreover, the oxidation signal of guanine relative to ds-DNA at the surface of Pt-Pd-CdO/SWCNTs/ds-DNA/GE has been considered as an bioelectroanalytical issue to monitoring cyanazine for the first time. Electrochemical impedance spectroscopic (EIS) signals have confirmed that the inclusion of Pt-Pd-CdO/SWCNTs at the surface of the GE has lowered chargetransfer resistance by ca.1.54 times and created a highly conductive state for monitoring of cyanazine in nanomolar concentration. On the other hand, differential pulse voltammograms (DPV) of Pt-Pd-CdO/SWCNTs/ ds-DNA/GE have indicated a linear dynamic range of 4.0 nM-70 mu M with a detection limit of 0.8 nM to the monitoring of cyanazine. In addition, the molecular docking study has emphasized that cyanazine herbicide is capable of binding to ds-DNA preferably at the guanine-cytosine rich sequences, and confirmed experimental results. In the final step, Pt-Pd-CdO/SWCNTs/ds-DNA/GE has been successfully utilized for the monitoring of cyanazine herbicide in food and water samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据