期刊
JOURNAL OF FOOD QUALITY
卷 2022, 期 -, 页码 -出版社
WILEY-HINDAWI
DOI: 10.1155/2022/9119547
关键词
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资金
- China Scholarship Council-University of Leeds Scholarship and Technological Innovation Service Capacity Building-Basic Scientific Research Expenses [PXM2020_014213_000017]
The study found that nobiletin can enhance endothelial cell function under nonstimulated and inflammatory conditions by upregulating eNOS and antioxidant genes while downregulating ET-1, showing a promising protective effect for cardiovascular health, likely through the NO/ET-1 dynamics and Nrf2 signaling pathway.
Nobiletin, a natural polymethoxylated flavonoid compound, has been shown to exert a wide range of biological activities. However, there is limited evidence on the molecular mechanisms by which nobiletin modulates endothelial cell function. Our aim was to investigate the potential of nobiletin to enhance endothelial cell functionality under nonstimulated and inflammatory conditions. To this end, gene expression relevant to nitric oxide (NO) production, endothelin 1 (ET-1), and antioxidant status in human endothelial cells EA.hy926 was determined using real-time PCR. The fluorescence probe 4,5-diaminofluorescein (DAF-2) was used to measure NO production. The results demonstrated significant upregulation of eNOS and antioxidant genes as well as downregulation of ET-1 by nobiletin under nonstimulated and TNF-alpha-stimulated inflammatory conditions. These findings suggest a promising protective effect of nobiletin with relevance for cardiovascular health, likely through the NO/ET-1 dynamics and Nrf2 signaling pathway.
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