期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 73, 期 8, 页码 2601-2617出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erac011
关键词
Arabidopsis thaliana; endoplasmic reticulum stress; GAAPs; glutathione; reactive oxygen species; unfolded protein response
资金
- National Natural Science Foundation of China [31670271]
This study reveals that GAAP1 and GAAP4 play redundant positive roles in regulating ER stress response in plants. They maintain proteostasis by inhibiting ROS accumulation through regulating glutathione content, thus enhancing cell viability and survival.
Maintaining proteostasis in the endoplasmic reticulum (ER) is critical for cell viability and plant survival under adverse conditions. The unfolded protein response (UPR) pathways interact with reactive oxygen species (ROS) to precisely trigger adaptive outputs or cell death under ER stress with varying degrees. However, little information is known about the relationship between UPR signalling and ROS regulation. Here, Arabidopsis GOLGI ANTI-APOPTOTIC PROTEIN1 (GAAP1)-GAAP4 were found to play redundant positive roles under ER stress. Genetic analysis showed that GAAP4 played a role in INOSITOL-REQUIRING ENZYME (IRE1)-dependent and -independent pathways. In addition, GAAPs played negative roles to activate the adaptive UPR under conditions of stress. Quantitative biochemical analysis showed that mutations in GAAP genes decreased the oxidised glutathione content and altered the pattern of ROS and glutathione in early ER stress. When plants were challenged with unmitigated ER stress, mutations in GAAP advanced ROS accumulation, which was associated with a decline in adaptive UPR. These data indicated that GAAPs resist cell death by regulating glutathione content to inhibit ROS accumulation and maintain UPR during ER stress. They provide a basis for further analysis of the regulation of cell fate decision under ER stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据