Sinapis alba L. and Triticum aestivum L. as biotest model species for evaluating municipal solid waste leachate toxicity

The volume of municipal solid waste (MSW) inputs is rapidly increasing with a growing human population, and its composition is changing due an increased diversity of materials being deposited. There is an associated increase in leachate, a common toxic byproduct of MSW facilities that must be collected and treated prior to its release into the environment. There is growing interest in plant-based methods that are economical and efficient for leachate toxicity assessment such as biological tests that use indicator species. In the present study, the tolerance thresholds of two herbaceous species, Sinapis alba L. (mustard) and Triticum aestivum L. (wheat) to increasing shares of leachate sourced from an MSW facility in the Czech Republic were assessed through a variety of physiological parameters. Soil-based biotests showed a stimulation in the shoot biomass, leaf expansion, primary root elongation and carbon assimilation rate of the selected plant species to leachate concentrations between 20 and 50 %. Higher leachate concentrations led to reductions in most physiological parameters, especially the elongation of seedling roots when growth solutions with >50 % leachate were applied. While S. alba was more sensitive to increasing proportions of leachate in terms of growth parameters of the shoot tissues, photosystem II efficiency and chlorophyll pigment concentrations were more responsive in T. aestivum, indicating species-dependent differences. The present biotests provide further support for the use of both Sinapis alba L and Triticum aestivum L. as indicator species of phytotoxicity.

Automated summary

With the increasing human population, the volume of municipal solid waste inputs is rapidly increasing, leading to a rise in leachate that needs to be collected and treated. Plant-based methods for leachate toxicity assessment are becoming more popular, and this study evaluated the tolerance thresholds of two herbaceous species to varying proportions of leachate.