Effect of Maleic Acid Root Conditioning on Release of Transforming Growth Factor Beta 1 from Infected Root Canal Dentin

Introduction: Chemically released growth factors play a vital role in regenerative endodontics. Transforming growth factor beta 1 (TGF-I31) is 1 of the most extensively studied bioactive molecules that promotes cell proliferation, differentiation, and chemotaxis. The goal of the current research was to analyze the effect of 7% maleic acid (MA) root conditioning of an infected root canal on the release of TGF-I31. Methods: Single-rooted human teeth were decoronated, and the canals were enlarged with a Peeso reamer. The samples were divided into biofilm and nonbiofilm groups. Subsequently, all the samples of both groups were flushed with 10 mL of each irrigant, namely, 1.5% sodium hypochlorite (NaOCl), 7% MA, 17% EDTA, and a combination of 1.5% NaOCl with 17% EDTA or 7% MA, for 10 minutes each. TGF-I31 was estimated quantitatively using an enzyme-linked immunosorbent assay kit. Results: TGF-I31 release was lowest among the biofilm samples compared with nonbiofilm among all the groups. MA 7% with 1.5% NaOCl rendered higher amounts of growth factor release in contrast to the combination of 17% EDTA and 1.5% NaOCl in both the biofilm and nonbiofilm groups (P < .048). The nonbiofilm samples treated with 7% MA alone illustrated higher growth factor release compared with 17% EDTA only (P < .006), but there was no significant difference in growth factor release among the biofilm samples treated with 7% MA and 17% EDTA. Conclusions: Bacterial biofilms modified the release of TGF-I31. MA 7% was observed to be significantly more efficacious than 17% EDTA in TGF-I31 growth factor release from radicular dentin. (J Endod 2022;48:620-624.)

Automated summary

The objective of this study was to analyze the effect of 7% maleic acid root conditioning on the release of TGF-I31 in infected root canals. The results showed that bacterial biofilms modified the release of TGF-I31, and 7% maleic acid exhibited significant efficacy in releasing TGF-I31 growth factor from radicular dentin.