A β-nicotinamide adenine dinucleotide electrochemical sensor based on polyoxometalate built by the combination of electrodeposition and self-assembly

beta-nicotinamide adenine dinucleotide (NADH), as vital coenzyme involved, is expected to be accurately detected in Clinical diagnosis and treatment. Polyoxometalates (POMs) have unique redox capacity with good electrochemical reversibility and fast electron transfer ability, which makes them become favorite materials for sensors. Here by using alternating electrodeposition and self-assembly techniques, Keggin-type polyoxometalates (PMo11V) were modified on glassy carbon electrodes with the help of polyallylamine hydrochloride (PAH), to construct a sensitive and stable electrochemical sensor for detection of NADH. The proposed (PAH/PMo11V)(4)/GCE was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) measurements and amperometrics method (i-t). The obtained electrode has high stability, good reproducibility, outstanding sensing capability for NADH, with a low detection limit of 1.9 x 10(-8) M, a wide linear range from 3.2 x 10(-7) to 8.2 x 10(-5) M and 1.1 x 10(-5) to 1.01 x 10(-3) M. This strategy, overcoming the drawbacks of POMs and solving the shortcomings that NADH requires high electro-catalytic potential, is simple in operation, low in cost, and could be widely used in electrochemisty field.

Automated summary

In this study, a sensitive and stable electrochemical sensor for the detection of beta-nicotinamide adenine dinucleotide (NADH) was constructed using polyallylamine hydrochloride (PAH) modified Keggin-type polyoxometalates (PMo11V). The sensor exhibited high stability, good reproducibility, and outstanding sensing capability for NADH, making it ideal for clinical diagnosis and treatment.