期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 905, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2021.115979
关键词
GR5 DNAzyme; Ti3C2Tx Mxenes; Label-free; Electrochemical biosensor; Pb2+ ion
资金
- National Key Research and Devel-opment Program of China [2016YFD0501208]
- Project of the Priority Academic Program Development of Jiangsu Higher EducationInstitutions (PAPD)
- Key Research and Development Program of Yangzhou City [YZ2017081]
In this study, a new electrochemical biosensor for Pb2+ detection was designed based on the interactions between nucleic acids and nanomaterials. The biosensor showed easy preparation, adsorptive tunability, and high sensitivity, making it suitable for potential applications in food safety inspection and environmental monitoring.
The interactions between nucleic acids and nanomaterials have been often utilized to design the electrochemical biosensor due to easy preparation, adsorptive tunability and high sensitivity. In this study, we have prepared a new electrochemical biosensor for Pb2+ detection based on GR5 DNAzyme/Ti3C2Tx Mxenes modified glassy carbon electrode (GR5 DNAzyme/Ti3C2Tx Mxenes-GCE). GR5 DNAzyme is an RNA-cleavage-type DNAzyme that shows high specificity towards Pb2+. With the presence of Pb2+, the cleavage of GR5 DNAzyme is activated at a ribo-adenine (rA) site in the substrate stranded DNA, leading to the unwinding of double-helix structure. This can enhance the adsorptive property of Ti3C2Tx Mxenes toward GR5 DNAzyme, which favors the ion intercalation into the Ti3C2Tx Mxenes and induces the increase of oxidation peak current. Therefore, we have successfully applied GR5 DNAzyme/Ti3C2Tx Mxenes-GCE to detect Pb2+ with a linear range from 0.5 to 32 nM and a detection limit of 0.1 nM. This electrochemical biosensor is simple, sensitive and selective for Pb2+ determination that it should find some potential applications in food safety inspection and environmental monitoring.
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